• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries
【2h】

A novel real-time polymerase chain reaction-based method for the detection and quantification of lactose-fermenting Enterobacteriaceae in the dairy and other food industries

机译：一种基于实时聚合酶链反应的新型方法，用于乳制品和其他食品工业中乳糖发酵肠杆菌科细菌的检测和定量

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The presence of lactose-fermenting Enterobacteriaceae and coliforms is routinely assessed to determine the hygienic quality of water and foods, particularly dairy products. This paper reports the use of lacZ-specific primers in an SYBR green I-based real-time PCR method for the easy and rapid detection of coliforms in dairy products. A large number of bacterial species were assayed to establish the specificity of the method. The sensitivity of the method was assessed using artificially contaminated cheeses. The limit of detection was 1 coliform cell in cheese samples enriched for 8. h in a culture medium. The entire procedure, including sample processing, enrichment, DNA extraction, and real-time PCR amplification, can be completed within 10 to 12. h, making it a single-day assay. © 2010 American Dairy Science Association.
机译：常规评估乳糖发酵肠杆菌科细菌和大肠菌的存在，以确定水和食品（尤其是乳制品）的卫生质量。本文报道了在基于SYBR green I的实时PCR方法中使用lacZ特异性引物，可轻松快速地检测乳制品中的大肠菌。分析了大量细菌物种以建立该方法的特异性。使用人工污染的奶酪评估了该方法的敏感性。检测极限为在培养基中富集了8 h的干酪样品中的1个大肠菌群细胞。整个过程（包括样品处理，富集，DNA提取和实时PCR扩增）可在10到12小时内完成，使其成为单日测定。 ©2010美国乳业科学协会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号